Deep proteomic profiling of vasopressin-sensitive collecting duct cells. I. Virtual Western blots and molecular weight distributions
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Deep proteomic profiling of vasopressin-sensitive collecting duct cells. I. Virtual Western blots and molecular weight distributions.
The mouse mpkCCD cell line is a continuous cultured epithelial cell line with characteristics of renal collecting duct principal cells. This line is widely used to study epithelial transport and its regulation. To provide a data resource useful for experimental design and interpretation in studies using mpkCCD cells, we have carried out "deep" proteomic profiling of these cells using three leve...
متن کاملCALL FOR PAPERS Omics and Epithelial Cell Biology Deep proteomic profiling of vasopressin-sensitive collecting duct cells. I. Virtual Western blots and molecular weight distributions
Yang CR, Tongyoo P, Emamian M, Sandoval PC, Raghuram V, Knepper MA. Deep proteomic profiling of vasopressin-sensitive collecting duct cells. I. Virtual Western blots and molecular weight distributions. Am J Physiol Cell Physiol 309: C785–C798, 2015. First published August 26, 2015; doi:10.1152/ajpcell.00213.2015.—The mouse mpkCCD cell line is a continuous cultured epithelial cell line with char...
متن کاملDeep Proteomic Profiling of 3 Vasopressin - Sensitive Collecting Duct Cells
6 Chin-Rang Yang, Viswanathan Raghuram, Milad Emamian, 7 Pablo C. Sandoval and Mark A. Knepper‡ 8 9 10 Epithelial Systems Biology Laboratory 11 NHLBI, National Institutes of Health, 12 Bethesda, MD 20892-1603, USA 13 14 Running title: Vasopressin Signaling in Cultured mpkCCD Cells 15 16 ‡Correspondence to Mark A. Knepper, National Institutes of Health, Bldg. 10, Room 17 6N307, 10 CENTER DR, MSC...
متن کاملDeep Proteomic Profiling of Vasopressin-Sensitive Collecting Duct
August 18, 2015 1 2 Deep Proteomic Profiling of Vasopressin-Sensitive Collecting Duct 3 Cells. I. Virtual Western Blots and Molecular Weight Distributions 4 5 6 Chin-Rang Yang*, Pumipat Tongyoo^*, Milad Emamian*, Pablo C. Sandoval*, 7 Viswanathan Raghuram* and Mark A. Knepper*‡ 8 9 *Epithelial Systems Biology Laboratory, NHLBI, National Institutes of Health, 10 Bethesda, MD 20892-1603, USA 11 a...
متن کاملDeep proteomic profiling of vasopressin-sensitive collecting duct cells. II. Bioinformatic analysis of vasopressin signaling.
Vasopressin controls osmotic water transport in the renal collecting duct through regulation of aquaporin-2 (AQP2). We carried out bioinformatic analysis of quantitative proteomic data from the accompanying article to investigate the mechanisms involved. The experiments used stable isotope labeling by amino acids in cell culture in cultured mpkCCD cells to quantify each protein species in each ...
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ژورنال
عنوان ژورنال: American Journal of Physiology-Cell Physiology
سال: 2015
ISSN: 0363-6143,1522-1563
DOI: 10.1152/ajpcell.00213.2015